Proceedings of the National Institute of Sciences of India. August 1948

Stedman and Stedman were of the opinion that desoxyribose nucleic acid occurs not in the chromosomes but in the nuclear sap and that it is the new compound formed as a result of the interaction of the above with the fuchsin-sulphurous acid that stains the chromosomes. [...] Carr (1945) claims (1) that by changing the pH the colour of basic fuchsin could be changed (2) that the specific staining of the nucleus is the result of the destruction of the cytoplasmic structures and (3) that the staining of the chromosomes is a mere adsorption phenomenon. [...] The cells begin to settle at the bottom of the flask 15 to 20 minutes after the addition of fresh medium and at the stipulated interval the wort is carefully poured out and the layer of cells left at the bottom utilised for making smears. [...] Thus the variable factors like control of the quantity to be seeded and hence the time of termination of the logaritmic phase the composition of wort the age of material used for seeding and teperature are possible within limits. [...] Thus in the technique employed the cells would be in a phase of equilibrium at the end of 24 hours and replacement of the spent wort with fresh medium induces multiplication of all the resting cells which grow at almost the same rate.